Microbial flow cytometry
KMB020F
Microbial flow cytometry
Course description
Flow cytometry is a high-throughput technique for counting and studying cells in suspension by passing them through a laser and an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of up to thousands of cells per second. Flow cytometry is used for single-cell analysis of cell populations and monitoring of population phenotypic heterogeneity as well as for preparative sorting of individual cells and subpopulations. Flow cytometry have important applications in many fields including fundamental and applied microbiology, bioprocess monitoring, protein engineering, plant biology and marine biology.
Course content
In the course we will cover several applications of microbial flow cytometry and provide a clear view of what kind of information can be obtained, as well as how to analyze and interpret the data. Focus of the course will be on getting practical experience of flow cytometry for single-cell analysis of bacteria and yeast.
Course leader
Magnus Carlquist
Applied Microbiology
Department of Chemistry, Faculty of Engineering
Lund University
More information
Magnus Carlquist
Tel: +46 46 222 98 75
Email: magnus.carlquist@tmb.lth.se