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Diagnostic PCR

Project responsibles
Prof. Peter Rådström
Dr. Jenny Schelin

Implementation of the Polymerase Chain Reaction (PCR) technology as a rapid microbiological detection method for effective control measures in primary production and further processing requires knowledge about the identity of PCR inhibitors, the mechanism of PCR inhibition, pre-PCR processing and how to quantify microorganisms in biological samples with real-time PCR. The concept of integrating sampling, sample treatment and the chemistry of PCR, i.e. pre-PCR processing, is addressed as a general approach to overcoming real-time PCR inhibition and producing samples optimal for PCR analysis. The goal of this research program is to improve and facilitate the implementation of PCR-based diagnostics in clinical diagnostics, food analysis and forensic analysis.

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